The purpose of this study was to compare college student’s Physical Self-Perception Profile (PSPP) (18) scores in female kinesiology majors and non-kinesiology majors. Participants included 68 female kinesiology majors and 88 female non-majors in a mid-sized university. The mean age for the kinesiology majors was 20.8 years with a standard deviation of 2.31 and non-kinesiology majors was 19.7 years with a standard deviation of 3.16. MANOVA results indicated a significant difference between kinesiology majors and non-kinesiology major’s self-perceptions. Results show that kinesiology majors had significant higher self-perceptions of their sports competence, physical condition, physical self-worth, and physical strength. Researchers believe that identifying groups of people with low self-perceptions of theirphysical abilities and implementing strategies to improve these self-perceptions to increase physical activity levels may help in decreasing weight related health issues. This study will aid coaches, teachers, parents, athletic trainers, and health and fitness instructors in assessing individuals who struggle with low self-esteem in relation to their physical abilities and movements. Professionals will be encouraged to provide physical ability support and implement effective strategies to improve self-perceptions in order to increase physical activity levels.